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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
ANAL.P-333 |
| Subject |
Separation of lipoproteins and exosome in plasma using frit-inlet asymmetrical flow field-flow fractionation with multi-angle light scattering |
| Authors |
Young Beom Kim, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
|
| Abstract |
Lipoproteins transport hydrophobic molecules such as cholesterol in blood and are known to be associated with diseases such as arteriosclerosis. Lipoproteins are classified by high-density lipoprotein (HDL, 5~15 nm), low-density lipoprotein (LDL, 18~28 nm), very low-density lipoprotein (VLDL, 30~80 nm) according to its size and density. Exosomes are extracellular vesicles secreted by cells and their sizes are known as about 30~150 nm in diameter, which are larger than LDL but similar or slightly larger than VLDL. Exosomes are known to play an important role in the intercellular communication as a mediator of RNA, protein, and lipids, therefore, it can be expected to be utilized for disease prognosis and treatment. However, the separation of lipoproteins and exosomes from blood has not been studied extensively.
Field-flow fractionation (FFF) is capable of separating sample components from nano to micron size.
In this study, lipoproteins and exosomes from blood plasma samples were separated by frit-inlet asymmetrical flow field-flow fractionation (FI-AF4) using field programming and detected by UV spectrophotometer (UV) and multi-angle light scattering (MALS). After separation and fractionation by FI-AF4, lipoproteins and exosomes were confirmed by Western blot and transmission electron microscope (TEM). |
| E-mail |
bum5203@yonsei.ac.kr |
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122nd General Meeting of the KCS