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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
ANAL.P-335 |
| Subject |
Profiling of lipoproteins from postmenopausal patients with osteoporosis by asymmetrical flow field-flow fractionation and nUPLC-ESI-MS/MS |
| Authors |
Kang geun Lee, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
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| Abstract |
Pathological mechanism of osteoporosis is unknown, but osteocytes affected by oxidized lipids inside the body have been known as a major factor. Bone mineral density (BMD) reaches at a highest level in mid 30s and decreases slowly over time, meaning that symptoms are hard to be detected and already serious when found out. Lipids play important roles in signal transmission, cell formation, and various cellular processes and they are expected to be biomarkers of a number of diseases. Therefore, it is interesting to study relationships between lipids consisting lipoproteins in plasma and osteoporosis for early diagnosis. There are several studies about lipids and osteoporosis in the medical field which BMD showed a positive correlation with low-density lipoprotein (LDL) and an inverse correlation with high-density lipoprotein (HDL). However, none of them conducted quantitative and qualitative analysis at the molecular level in detail.
In this study, 39 plasma samples of woman were classified into three groups of patients in which women having osteoporosis with no clinical history (n=10), women having osteoporosis with history (n=7), age-matched control (n=22). Lipoproteins were first separated by sizes using asymmetrical flow field-flow fractionation and then lipids contained in HDL and LDL were analyzed by nanoflow ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (nUHPLC-ESI-MS/MS).
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| E-mail |
eagle52109@yonsei.ac.kr |
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122nd General Meeting of the KCS