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|
| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
ANAL.P-344 |
| Subject |
Development of a ganglioside profiling method by using a reversed phase liquid chromatography/mass spectrometry (RPLC/MS) platform |
| Authors |
Jun young Park, TaeMin Park, Youngjoo Kal, Sangwon Cha*
Department of Chemistry, Hankuk University of Foreign Studies, Korea
|
| Abstract |
Gangliosides are acidic glycosphingolipids which contain sugar chains with a variety of sialic acid residues. Gangliosides have several structural isomers of which glycan structures vary. However, most of reported reversed phase liquid chromatography/mass spectrometry (RPLC/MS) platforms could not separate these structural isomers. Recently, we have developed an RPLC method that could effectively separate the ganglioside isomers with a C18 column and an ammonium bicarbonate additive. In our previous study, the optimal salt condition which can give complete ganglioside isomer separation while maintaining high sensitivity was suggested. In addition, with an optimized RPLC/MS condition, we successfully profiled total gangliosides present in porcine brains with clear isomer differentiation. In this study, we further optimized our method by evaluating the limits of detection (LODs) of the major gangliosides, peak capacity, peak resolution, and so on. |
| E-mail |
pjy931119@naver.com |
|
122nd General Meeting of the KCS