122nd General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation for Young Scientist in Environmental and Energy Chemistry
Room No. Room 306A
Time THU 10:10-:
Code ENVR.O-3
Subject Effect of Inter-Enzyme Electrical Hindrance on Direct Electron Transfer at Synthetic Enzyme-Electrode Interface via Spatially Tunable-, and Nano-patterned Enzyme Electrode
Authors Hyeryeong Lee
Division of Environmental Engineering, Gwangju Institute of Science and Technology, Korea
Abstract Direct electron transfer (DET) in the enzyme-electrode system is crucial process for development of the alternative power sources of bioelectronics devices, biosensors, biofuel cells, etc. Efficient electrical communication in DET-based enzyme-electrode is important since DET efficiency of enzyme-electrode governs the performance of the devices. However, the enzymatic structure which the enzymatic active site is buried deep inside the protein matrix, obstruct the direct contact between enzymatic active site that is electron donor and electrode surface, causing poor electrical contact of enzymatic cofactor toward electrode surface. In addition to this, enzymatic agglomeration caused by protein-protein hydrophobic interactions could insulate DET during the immobilization of large amount of enzymes on the electrode. At the time, enzymatic cofactor which is electron donor for electrode can be hindered by non-conductive exterior of adjacent enzymes and ET at enzyme-interface will be prevented with its charge transfer resistance increased. Thus, in this study, the two factors that are ET distance and inter-enzyme steric hindrance, were considered to construct enzyme-electrode system with highly efficient DET process. Here, the synthetic glucose dehydrogenase (GDH) that DET capability and binding stability on the electrode. Furthermore, the influence of adjacent enzymes on DET efficiency was investigated on nano-patterned electrode, rendering nano-patterned enzyme-electrode where enzymes are spatially tuned on the electrode. The electrical performance of DET-based enzyme electrode was then estimated depending on condition of spatial tuning; less inter-enzyme insulation enabling favorable ET.
E-mail heather@gist.ac.kr