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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-416 |
| Subject |
Enhancing enantioselectivity of Candida antarctica lipase B towards but-3-yn-2-ol |
| Authors |
Seonghyeon Yi, Seongsoon Park*
Department of Chemistry, Sungshin University, Korea
|
| Abstract |
Candida antarctica lipase B (CAL-B) is one of the most widely used lipases for preparation of chiral building blocks because CAL-B possesses high enantioselectivity towards many secondary alcohols. However, CAL-B exhibits low enantioselectivity towards secondary alcohols bearing small substituents (i.e. but-3-yn-2-ol (E = 6) and 2-butanol (E = 7)). In this study, we present improvement of enantioselectivity of CAL-B toward but-3-yn-2-ol and 2-butanol. The medium binding pocket was altered to reduce the space of the medium binding pocket of CAL-B. The mutant enzymes exhibited higher enantioselectivity (E > 200 and E > 20) towards but-3-yn-2-ol and 2-butanol, respectively, compared to those of the wild type enzyme. |
| E-mail |
yksh19952@daum.net |
|
122nd General Meeting of the KCS