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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-422 |
| Subject |
Giant Unilamellar Vesicles with Actin Polymerization, Mimicry of Cellular Structure |
| Authors |
Sungwoo Jeong, Huong Thanh Nguyen, Agustina Setiawati1, Keel Yong Lee2, Kwanwoo Shin*
Department of Chemistry, Sogang University, Korea
1Department of Life Science, Sogang University, Korea
2School of Engineering and Applied Science, Harvard University, Korea
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| Abstract |
Mimicking the living cell requires diverse chemical components to handle. First, because the fundamental cellular structure requires cell membrane, we created Giant Unilamellar Vesicles (GUVs) similar sized as living animal cells (10μm~100μm) as an artificial cellular membrane. We used electroformation for creating GUVs, consisted with the most abundant unsaturated and saturated phospholipids in animal cells, DOPC, DOPS, DPPC, and cholesterol. To mimic the cytoskeletal structure of the cell, we used Rabbit Skeletal Muscle Actin for the G-actin, the essential multi-functional protein of all eukaryotic cells, to create actin filaments through actin polymerization. Actin polymerization requires several chemicals to be triggered. It essentially requires divalent cations and ATP. To have a continuous actin polymerization inside the GUVs, it requires the continuous divalent ions supply. As a result, we inserted ionophores (Mg2+ and K+) in GUVs for the ions supply. We simply encapsulate the G-actin monomers then observed the actin polymerization within GUV, triggered by the change of the Mg2+ concentration. These experiments demonstrate the possibility that could lead to the complete artificial creation of living cells structures. |
| E-mail |
jluke714@sogang.ac.kr |
|
122nd General Meeting of the KCS