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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-425 |
| Subject |
Assessment of Avian Influenza Virus vs Host-cell Infection Profiling by a Superoxide Detecting Agent (SoDA-1) |
| Authors |
Md Mamunul Haque, Se-young Jang, Dhiraj Murale1, Seong Cheol Hong, Jun-Seok Lee*
Molecular Recognition Research Center, Korea Institute of Science and Technology, Korea
1Molecular Recognition Research Center, Korea Institute of Science and Technology, India
|
| Abstract |
Avian Influenza (AI) virus caused an annual epidemic outbreak that led to destroy millions of poultry, and over 1500 human infection for serious illness or even death. However, studies of AI virus infection in the host have been hampered due to the complex propagation mechanism of the virus, and high rate of mutations during AI replication. In addition, susceptibility of AI virus differs depending on genetic backgrounds of hosts. Till now, research has been carried out for the rapid diagnosis of the viral infection. The existing gold‐standard diagnosis method is the culturing of viruses in a chicken embryonated egg for days followed by a hemagglutination assay. Positive samples from this assay is subsequently analyzed by immunoblotting or PCR sequencing to confirm subtypes. This whole method requires specialized facilities to handle large number of pricey embryonated eggs, and costly reagents. Moreover, fast mutation of AI virus leads to antigenic drift that significantly hamper the antibody-based assay. Therefore, a fast and simple surveillance method for AI infection is crucially required. Here, a differential sensing approach to distinguish AI subtypes using cell lines and fluorescent sensors was demonstrated. Different organs originated cell lines might have significantly different susceptibility against AI viruses, since host cells consist of diverse proteins or surface glycan structures. To quantify AI infection, a highly cell‐permeable fluorescent sensor was designed, which reacts with superoxide that is an upstream product of AI infection as host cell defense mechanism. This differential sensing strategy successfully proved discriminations of AI subtypes and demonstrated as a useful primary screening platform to monitor a large number of samples. |
| E-mail |
mamun@chembiol.re.kr |
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122nd General Meeting of the KCS