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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-426 |
| Subject |
Direct observation of DNA substitutive mutations in human cells by CRISPR-Cas9 endonuclease |
| Authors |
Gue ho Hwang, Jihyeon Yu1, Sangsu Bae2,*
Chemistry, Hanyang University, Korea
1Department of chemistry, Hanyang University, Korea
2Department of Chemistry, Hanyang University, Korea
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| Abstract |
CRISPR-Cas9 is used to immune system resisting external plasmid and viruses in prokaryotes by degrading the invading nucleic acids. CRISPR-Cas9 is used for a powerful genome editing tools by taking the function of nuclease. After CRISPR-Cas9 binds the target DNA sequence, CRISPR-Cas9 makes a double-stranded break (DSB) in specific region. The DSB is repaired by non-homologous end joining (NHEJ) and homology directed repair (HDR) systems. HDR recovers the DNA sequence perfectly, but NHEJ can make insertions, deletions and substitutions. The analysis of substitutions is difficult, because the substitutions also can be produced by PCR and next generation sequencing (NGS) error. So, we develop the analysis method for substitution and analyze the pattern of substitutions at 98 samples. |
| E-mail |
iamleohwang@gmail.com |
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122nd General Meeting of the KCS