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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-428 |
| Subject |
Target-specific Color Development
for Food-residential Bacteria Detection by Low-cost
3D Paper Device with Cocktailing Chromogenic Substrates.
|
| Authors |
Hyeok Jung Kim, Hyeran Noh1,*
Department of optometry, Seoul National University of Science & Technology, Korea
1Department of Optometry, Seoul National University of Science & Technology, Korea
|
| Abstract |
Enzymatic substrate has been regarded as one of powerful tools for quantification and evaluation of bacterial activity. Here, we investigated chromogenic substrates for four strains of pathogenic bacteria, Escherichia coli, Escherichia coli O:157, Salmonella spp., Staphylococcus, with collaborating paper-microfluidic devices. The developed three-dimensional paper sensor consisted of multilayered sheets of wax-patterned paper, cocktailed chromogenic substrates, lysis and oxidation agent. Each mixed enzymatic substrate mixture offered specificity on the target bacterium with showing four-different colors. Without enhancement step, the LOD below 108 cfu/mL was demonstrated with 60 min of analysis time. With enhancement treatment for 4 hours, the lower number of bacteria (~ 10 cfu/mL) was detected. When spiked into food sample with enhancement for 8 hours, the specimens were sensitively (10 cfu/mL) detected by image analysis and naked eye observation both. The compact, free-equipment and easy-to-use paper sensor is expected to show utility in first screening detection and quantitative analysis of foodborne pathogen. |
| E-mail |
awct1004@gmail.com |
|
122nd General Meeting of the KCS