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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
ANAL.P-371 |
| Subject |
Highly sensitive molecular diagnostics using SERS-PCR |
| Authors |
Yixuan Wu, Jaebum Choo1,*
Bionano Technology, Hanyang University, Korea
1Department of Bionano Engineering, Hanyang University, Korea
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| Abstract |
Recently, quantitative real-time PCR (QPCR) have been extensively used for the diagnosis of a wide range of infectious diseases. This technique found valuable clinical application in the diagnosis of viral diseases. However, QPCR suffers from several problems including time-consuming process and high fluorescent background signal. In the present work, we developed a novel molecular diagnostic method using SERS-PCR for the sensitive and specific detection of target genes. Due to the characteristics of amplifying Raman signals by factors up to 10-14 orders of magnitude and unique molecular fingerprinting information, surface-enhanced Raman scattering (SERS)-based biosensors provide ultrahigh sensitivity and have more advantages over the fluorescent detection method. In the present study, we employed an asymmetric-PCR to amplify the target gene in a short time, and then a SERS detection technique has been used for its highly sensitive detection. By combining the PCR and SERS as a detection tool, it would prove to be effective for the rapid and sensitive detection of a low concentration of target.
References
1. Kneipp, K., Wang, Y., Kneipp, H., Perelman, L. T., & Itzkan, I, Phys. Rev. Lett. 78, 1667 (1997).
2. Wu, Y., Jiang, T., Wu, Z., & Yu, R, Biosens. Bioelectron, 99, 646-652 (2018).
Keywords: SERS-PCR, Pseudomonas aeruginosa, Ratiometirc gene detection, molecular diagnostics
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| E-mail |
wuonexuan@hotmail.com |
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122nd General Meeting of the KCS