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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-431 |
| Subject |
Comprehensive comparison of Cas9 and Cpf1 in human cell |
| Authors |
Woojae Son, Sangsu Bae*
Department of Chemistry, Hanyang University, Korea
|
| Abstract |
CRISPR-Cas, an intrinsic immune system of bacteria, has been found in a number of bacterial species, among which two RNA-guided endonuclease, Cas9 and Cpf1, are now widely used as powerful genome editing tools. Because Cas9 and Cpf1 have unique characteristics and need to be compared to which CRISPRs are more suitable for genome editing tools, we chose an endogenous region where both CRISPR systems in human cells can target. In addition, the cleavage regions of two CRISPR were matched to make more clear comparison. We compared the success rate (> 1%), average mutation ratio, indel pattern, and KI efficiency (ssODN, HDR, and NHEJ-mediated K.I) of SpCas9, AsCpf1 and LbCpf1 in these 68 selected endogenous targets. These comprehensive comparisons will allow us to show which CRISPRs are most effective as a genome engineering tool. |
| E-mail |
woojae00@naver.com |
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122nd General Meeting of the KCS