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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
ANAL.P-373 |
| Subject |
Disposable HPLC micro-column with a welded stainless-steel frit at the column outlet and a silver cemented frit at the column inlet |
| Authors |
Miri Mun, Won jo Cheong*
Department of Chemistry, Inha University, Korea
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| Abstract |
In this study, we have developed a disposable microcolumn with a welded metal frit and a silver cement frit. First, one end of 30 cm glass-lined stainless-steel tubing (1.6 mm od, and 0.5 mm id) was welded with a stainless-steel screen frit of 1.6 mm diameter using a micro-welding machine. Next, the column was connected to a slurry packer and packed with C18 bound partially sub-2 µm silica monolith particles. Next, a silver cement frit was made inside the column by injecting the reaction mixture into the packed column. Silver is reduced on the particle surface and in an empty space among the particles, forming a solid silver network structure at the column inlet area. This silver cemented frit can hold the stationary phase inside the column even at a high flow rate. The column was tested for separation of a test mix composed of phenol, acetophenone, 4-methyl-2-nitroaniline, benzene and toluene. The chromatographic performance of this column (with a metal welded frit and a silver cemented frit) was compared with another column (same dimension) with two screen frits packed with the same stationary phase. The columns of a welded frit and a silver cemented frits showed ca. 15 % better separation efficiency (number of theoretical plates) than those of two screen frits. This study shows a promising vision for realization of disposable micro-columns. |
| E-mail |
ml1042@naver.com |
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122nd General Meeting of the KCS