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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-436 |
| Subject |
Development of ssDNA aptamer for diagnosis and inhibition of highly pathogenic avian influenza virus subtype H5N1 |
| Authors |
Jae Woo Choi, A-Ru Kim, Sang-Heon Kim, Songa Park1, Moon-Young Yoon*
Department of Chemistry, Hanyang University, Korea
1DEPARTMENT OF CHEMISTRY, Hanyang University, Korea
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| Abstract |
Avian influenza viruses have devastated the poultry industry in numerous countries irrespective of industrial development resulting in the deaths of millions of birds. Highly pathogenic avian influenza (HPAI) is characterized by high mortality and ability to spread directly from birds to humans. The early diagnosis of avian influenza has been difficult because of its variations in the pathogenicity and the genetic diversity of each virus subtype. Therefore, it is urgent to develop a sensitive and accurate virus diagnosis system. We developed ssDNA aptamer as a small probe to diagnose avian influenza. Through 6 rounds of SELEX towards one of highly pathogenic subtype virus (H5N1), we identified 16 aptamer sequences and selected 2 sequences based on higher frequency. The two selected aptamers showed strong binding affinities and low detection limits. We found that it could bind more specifically to H5N1 compared to other avian influenza virus subtypes. Furthermore, our aptamer inhibited the aggregation of red blood cell which is called hemagglutination. Though more research is needed such as binding site or way of signal translation, our results showed that the screened aptamers worked well as a small molecular probe towards H5N1. It will stand livestock industry in good stead by applying to sensitive diagnosis or suppression of avian influenza. |
| E-mail |
ddd759@naver.com |
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122nd General Meeting of the KCS