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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-442 |
| Subject |
Method for identifying lethality of a mutation gene |
| Authors |
Se eun Park, Sangsu Bae*
Department of Chemistry, Hanyang University, Korea
|
| Abstract |
CRISPR-Cas system consists of a guide RNA that specifically binds to a specific nucleotide sequence and Cas9 nuclease, which acts as a scissor to cut a specific sequence. By using CRISPR-Cas system, we can make cell lines in which the function of specific genes is knocked out. However, during the course of the experiment, the cell line continued to die during single cell culture, and all the alleles could not continue to obtain the knock-out cell line. The Knock-out cells that died and could not be obtained are due to loss of function of essential genes for survival. Here, we describe the lethality test for cells with knock-out of specific genes, using Next generation sequencing (NGS). Using our method, it can help to know whether the knock-out of a specific gene affects the lethal of the cell, and to avoid repeated experiment failures making knock-out cell line. |
| E-mail |
seuni001@naver.com |
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122nd General Meeting of the KCS