122nd General Meeting of the KCS

Type Poster Presentation
Area Medicinal Chemistry
Room No. Grand Ballroom
Time 10월 18일 (목요일) 11:00~12:30
Code MEDI.P-314
Subject Anti-Atherosclerotic and Lipid-Lowering Effects of the Mixture of Crataegus pinnatifida Fruit and Lycopus lucidus
Authors Jong-Min Han*, Gyeongtea Ban1
Life Science, Daejeon University, Korea
1Dae Han Bio Pharm Inc., Korea
Abstract Development of atherosclerosis is caused by a high concentration of LDL‐cholesterol in the blood. This study was to investigate the possibility of the lipid-lowering and anti-atherosclerotic effects of the mixed extracts of Crataegus pinnatifida Fruit and Lycopus lucidus (MCL) in human umbilical vein endothelial cells (HUVECs) and Poloxamer-407 (P-407) mouse model, and to explore its underlying mechanism. For confirmation of reproducibility of MCL, we conducted a fingerprint using high performance liquid chromatography (HPLC)-based compositional analysis, with four main compounds including protocatechuic acid, quercetin dehydrate, chlorogenic acid, and cirsilineol respectively. In HUVECs, MCL inhibited tumor necrosis factor (TNF)-α-induced THP-1 monocytes adhesion to the HUVECs as well as up-regulation of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). In mouse models, serum lipid levels (total cholesterol, TC; triglycerides, TG; and low-density lipoprotein cholesterol, LDL-C) were increased significantly in the P-407-induced group as compared to the normal group. Interestingly, MCL administration caused a significant reduction in serum TC (p<0.01) and TG (p<0.01) levels. These results indicate that MCL can function as a lipid-lowering agent and prevents hyperlipidemic atherosclerosis through attenuation of adhesion molecule expression.
E-mail malius@dju.kr