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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists I |
| Room No. |
Room 321 |
| Time |
THU 09:10-: |
| Code |
ANAL1.O-2 |
| Subject |
Development of iCLEM for real-time observation and easy sample replacement. |
| Authors |
GwangHoon Lee, Boklae Cho1,*
Charged Particle Beam Research Institute, ModuleSci Co., Ltd., Korea
1Korea Research Institute of Standards and Science, Korea
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| Abstract |
Quantum dots and light emitting diodes (LEDs) are normally studied by using an light / fluorescence microscope (LM / FM) due to their luminescent properties. However, when a problem occurs in a device, the defect is often much smaller than a micron, making it difficult to identify its origin with a general light microscope. To overcome these limitations, an electron microscope with higher resolution than an light microscope should be used. The CLEM(Correlative Light and Electron Microscope) is a device that allows a sample holder to be shared by two microscopes and can perform high magnification imaging using an electron microscope. However, the sample can be deformed or contaminated during the transportation of the holder between the equipments. In order to overcome these drawbacks, various attempts have been made to develop an iCLEM (integrated CLEM).
We have developed two kinds of iCLEM consisting of a upright type and an inverted type, maximizing system scalability and accessibility. The upright iCLEM can acquire light / fluorescence images and scanning electron microscope images of an optically opaque sample simultaneously by using a light mirror with a center hole. The inverted iCLEM can image an optically transparent nano-bio sampled through a sample holder where the sample is loaded on ITO (Indium Tin Oxide) coated glass. The iCLEM can acquire fluorescence information. We are now constructing a platform that combines iCLEM with EDS, Raman spectroscope, and CL (Cathode-Luminescence) detector. In this presentation, we will discuss the principles and structure of the iCLEM and show the images to discuss future applications. |
| E-mail |
ghlee@modulesci.com |
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122nd General Meeting of the KCS