122nd General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Grand Ballroom
Time 10월 19일 (금요일) 11:00~12:30
Code LIFE.P-447
Subject Hydroxyapatite-Assisted Enhancement of Subtilisin Activity
Authors Lee Yoonjae, Seongsoon Park1,*
chemistry, Sungshin University, Korea
1Department of Chemistry, Sungshin University, Korea
Abstract In general, the activity of biocatalyst decreases in organic solvent. Subtilisin (protease from Bacillus licheniformis), which is one of the widely used enzymes in industry, also exhibits low activity in organic solvents. However, it has been known that lyophilization of subtilisin with KCl salt dramatically improves subtilisin activity in organic solvents by up to a factor of 100. Although KCl salt is useful to improve subtilisin activity, there are a few drawbacks, such as difficulties of controlling moisture contents and recycling the enzyme, for the application of organic synthesis. In the current study, hydroxyapatite has used as a supporting material for subtilisin immobilization. Hydroxyapatite is one of the main components of the biological bone system, and is non-toxic and biocompatible. A series of the lyophilized forms (2-20%) of subtilisin with hydroxyapatite were prepared and their activities were compared with that of the free form of subtilisin in organic solvents such as hexane, isopropyl ether, and THF. The immobilized subtilisin on hydroxyapatite exhibited the comparable activity to that of the lyophilized form of subtilisin with KCl and can be recycled several times.
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