122nd General Meeting of the KCS

Type Poster Presentation
Area Medicinal Chemistry
Room No. Grand Ballroom
Time 10월 18일 (목요일) 11:00~12:30
Code MEDI.P-318
Subject Ginsenoside Rb2 suppresses multiple mechanisms of glutamate mediated neurotoxicity in HT22 cells
Authors Dong Hoi Kim, Heesu Lee1, Ae Nim Pae2, Jae Wook Lee3,*
Research Center for Dementia DTC, Korea Institute of Science and Technology, Korea
1Department of Dentistry, Gangneung-Wonju National University, Korea
2Korea Institute of Science and Technology, Korea
3Convergence Research Center for Dementia DTC, Korea Institute of Science and Technology, Korea
Abstract The neuroprotective effects of ginsenosides were evaluated using glutamate induced neurotoxicity model. Among the ginsenosides, Rb2 shows protective effect against glutamate mediated neuronal cell death using HT22 cells. To investigate mode of action of Rb2 in neuroprotective effect, we analyzed cellular Ca2+ and reactive oxygen spices (ROS) level, which is trigger to apoptosis through activation of MAPK and AIF transloaction. Image analysis using DCFDH and Flu3 shows Rb2 block Ca2+ influx and accumulation of cellular ROS. Western blot analysis shows that Rb2 inhibits MAPK activation and AIF translocation by initiating glutamate induced oxidative stress. Further animal study using the Mongolian gerbil of ischemic brain injury mouse model also shows Rb2 strongly inhibits brain ischemic injury. We currently undertake structural elaboration to improve Rb2 delivery to brain.
E-mail 091482@kist.re.kr