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|
| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-450 |
| Subject |
Expression and characterization of a novel carbohydrate esterase SmAcE1 from Sinorhizobium meliloti suitable for biodiesel synthesis. |
| Authors |
Ly Thi Huong Luu Le, Doo Hun Kim*
Department of Chemistry, Sookmyung Women's University, Korea
|
| Abstract |
Biocatalytic synthesis is a promising environmentally friendly process for the production of biodiesel, a sustainable alternative fuel from renewable plant resources. Here, SmAcE1 from Sinorhizobium meliloti, a gram-negative nitrogen fixing soil bacteria, was identified, purified, characterized and used for synthesis of biodiesel products. SmAcE1 belonged to an α/β hydrolase fold in GDSL superfamily with catalytic triad Ser15, His195 and Asp192 and oxyanion hole-forming SGNH (Ser15, Gly57, Asn97 and His195). This enzyme was most active at pH 8.0 and it was stable up to 700C. SmAcE1 exhibited broad substrate specificity toward various p-nitrophenyl esters, lipids, carbohydrates and tertiary alcohols. Furthermore, SmAcE1 not only synthesized butyl acetate and ethyl acetate but also synthesized different types of oleates by esterification using oleic acid and short chain alcohols (methanol, ethanol and butanol). Therefore, SmAcE1 might be a good biocatalyst in ester synthesis in biodiesel industry. |
| E-mail |
ly.12000532@gmail.com |
|
122nd General Meeting of the KCS