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|
| Type |
Symposium |
| Area |
Recent Advances of Nanobiosensor for Detection of Disease Biomarkers |
| Room No. |
Room 321 |
| Time |
FRI 14:50-: |
| Code |
ANAL2-2 |
| Subject |
Towards Single-molecule Biosensors using Super-localization Fluorescence-free Nanoscopy |
| Authors |
Seungah Lee, Seong Ho Kang*
Department of Applied Chemistry, Kyung Hee University, Korea
|
| Abstract |
Nanoscopy based on single-molecule localization offers a practical way to explore plasmonic interactions with nanometer resolution. Over the last decade, two fields have dominated the attention of sub-diffraction photonics research: plasmonic and fluorescence nanoscopy. Fluorescence nanoscopy obstruct more sensitive and selective immunodetection due to their limitations associated with photo-stability. In contrast, plasmonic nanoscopy hold great promise for the robust quantitative nano characterization of functional nanomaterials without fluorescent labeling. We have developed high sensitive and precise nanoimmunosensors by 3D as well as 2D antibody-antigen binding on nanopatterened biochips using super-localization nanoscopy based on plasmon scattering. The biosensors have demonstrated the possibility for detection of extremely low concentration detection (i.e., yM) of various disease-related biomolecules at the single-molecule level. |
| E-mail |
moon1131@gmail.com |
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122nd General Meeting of the KCS