122nd General Meeting of the KCS

Type Award Lecture in Division
Area Generation Networking Symposium in Analytical Chemistry
Room No. Room 321
Time THU 17:10-:
Code ANAL1-5
Subject Mass spectrometry of surfaces tailored with biomolecules and nanostructures, and non-covalent complexes
Authors Sang Yun Han
Department of Nanochemistry, Gachon University, Korea
Abstract In this talk, development of mass spectrometric methods and applications that have not been in the regime of conventional mass spectrometry will be presented. (1) Mass spectrometry (MS) of surfaces tailored with biological molecules such as protein chips on which various antibodies are immobilized using self-assembled monolayers (SAMs) have been investigated as a new way of molecular diagnosis. In an effort to characterize SAMs on gold, cation-assisted laser desorption ionization (LDI) MS was developed. In the course of this study, the mechanism of MALDI for SAMs on Au was carefully elucidated. In addition, the method of microwave-assisted on-chip tryptic digestion for protein chips was further developed for rapid characterization of label-free detection of biochips. (2) Using nano-structured surfaces, the mechanism for matrix-free LDI process was investigated, which revealed rapid surface heating plays a key role in intact desorption of thermally labile molecules such as peptides. From this finding, by choosing surfaces of appropriate thermal property, LDI of large molecules was successfully demonstrated even without matrix or surface nanostructures. (3) Tandem mass spectrometry was further extended to understand intermolecular interactions such as ionic hydrogen bonding and hydrogen bonding involved in proton-bound dimers of nucleic acid bases and in G-quadruplexes. As a result, it was demonstrated that hydrogen bonding interactions that create formation of non-covalent complexes also plays an important role in determining the fate of collisionally activated complexes in dissociation. Accordingly, mass spectrometry is a powerful tool of which applications can be further extended beyond its conventional role of rapid characterization of large and complex molecules.
E-mail sanghan@gachon.ac.kr