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| Type |
Poster Presentation |
| Area |
Polymer Chemistry |
| Room No. |
Grand Ballroom |
| Time |
10월 18일 (목요일) 11:00~12:30 |
| Code |
POLY.P-34 |
| Subject |
Development of cyclic olefin copolymer-based immunoassay chips using functional polymer |
| Authors |
Daekyung Sung
Center for Convergence Bioceramic Materials, Korea Institute of Ceramic Engineering and Technology, Korea |
| Abstract |
We developed cyclic olefin copolymer-based immunoassay chips through pre-activated polymer adlayers. For the surface modification of COC substrates we synthesized two types of amphiphilic polymers having three important functions: hydrophobic groups, a PEG component, and an NHS ester group for conjugation of biomolecules. Formation of the polymer adlayers on COC surface was confirmed using a contact angle analyzer. Sandwich immunoassay using anti-rabbit IgG immobilized COC surface showed linear response to rabbit IgG of model analyte over a range from 1 to 1000 ng/mL with detection limit of 1–10 ng/mL. We also fabricated a Lab-On-a Chip type of COC biochip, which could detect a cardiac marker protein, troponin I (TnI), with detection limit of 10 ng/mL. |
| E-mail |
dksung@kicet.re.kr |
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122nd General Meeting of the KCS