|
Type |
Symposium |
Area |
Recent Trends in Genome Editing Technique |
Room No. |
Room 314 |
Time |
FRI 10:15-: |
Code |
LIFE2-4 |
Subject |
Development of multi-functional guide RNA for gene manipulation using Cas9 and Cpf1 |
Authors |
Yongsub Kim Department of Biomedical Sciences, University of Ulsan College of Medicine, ASAN Medical Center, Korea |
Abstract |
Engineered nucleases that allow targeted mutagenesis in living cells and organisms are broadly useful in biological research. Engineered nucleases induce target specific DNA double-strand breaks (DSBs) and DSBs subsequently recruit intracellular machinery for either non-homologous end-joining (NHEJ) or homology-directed repair (HDR) to the DSB site to mediate genome editing. We have developed ZFNs, TALENs, CRISPR-mediated Cas9 and Cpf1 to modify chromosomal DNA in living cells. Using these engineered nucleases, we also generated several knockout cell lines and mode organisms with high efficiencies. Also, we have developed guide RNA variants for multiplex genome engineering & orthogonal gene manipulation. These constructs will expand the use of genome editing tools in biomedical research. |
E-mail |
Yongsub1.kim@gmail.com |
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