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| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Biological Chemists |
| Room No. |
Room 314 |
| Time |
THU 09:40-: |
| Code |
LIFE.O-3 |
| Subject |
Single Molecule Approach for Cell Biology;
Control of Notch and Cadherin Signaling at Single Cell Level |
| Authors |
Daeha Seo
Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science & Technology, Korea |
| Abstract |
Tools capable of imaging and perturbing mechanical signaling pathways with fine spatiotemporal resolution have been elusive despite their importance in diverse cellular processes. The challenge in developing a mechanogenetic (i.e. genetically encoded mechanical perturbations) toolkit stems from the fact that many mechanically-activated processes are localized in space and time, yet additionally require quantitative mechanical loading to become activated. To address this unmet need, we synthesized magnetoplasmonic nanoparticles that can image, localize, and mechanically load targeted proteins with high spatiotemporal resolution. We demonstrate their utility as a quantitative perturbation system by investigating the cell surface activation of Notch and E-cadherin receptors. By measuring cellular responses to various spatial, chemical, temporal, and mechanical inputs at the single molecule and single cell level, we reveal how spatial segregation and mechanical force cooperate to direct receptor activation dynamics. This generalizable technique can be used to control and understand diverse mechanosensitive processes in cell signaling.
General References
(1) Daeha Seo, Kaden M. Southard, Ji-wook Kim, Hyunjung Lee, Justin Farlow, Jung-uk Lee, David Litt, Thomas Haas, Jinwoo Cheon, A. Paul Alivisatos, Zev J. Gartner, Young-wook Jun “A Mechanogenetic Toolkit for Interrogating Cell Signaling in Space and Time” Cell, 2016, 165, 1507.
(2) Ji-wook Kim, Daeha Seo, Jung-uk Lee, Kaden M. Southard, Yongjun Lim, Zev J. Gartner, Young-wook Jun, Jinwoo Cheon “Single cell mechanogenetics using monovalent magnetoplasmonic nanoparticles” Nature Protocol, 2017, 12, 1871
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| E-mail |
livewire@dgist.ac.kr |
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122nd General Meeting of the KCS