122nd General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room 321
Time FRI 10:45-:
Code ANAL2.O-18
Subject Analysis of characteristic serum lipids of post-hepatectomy liver failure from partially hepatectomized swine using nanoflow UHPLC-ESI-MS/MS
Authors HaeA Kim, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Abstract Since liver possesses the big capacity to regenerate, hepatectomy is a common treatment for the disease like hepatocellular carcinoma. However, liver resections are complex operations and have a risk of complication. Especially, post-hepatectomy liver failure (PHLF) which is a liver failure after operation is one of the most serious complications and a big part of the reason of death. Liver has an important role in lipid metabolism. Therefore if liver doesn’t function properly, the homeostasis of lipids might be failed. In this study, the changes of lipid profiles of swine serums were observed depending on rate of hepatectomy and time after operation using nanoflow ultrahigh-pressure liquid chromatography electrospray ionization tandem mass spectrometry (nUHPLC-ESI-MS/MS) to give more accurate insight into PHLF. The swines were divided into fake operation group (sham, n=6), 70% partial hepatectomy group (70% PHx, n=7), and 90% partial hepatectomy group (90% PHx, n=7). The serums were collected before operation, and 14 hours, 30 hours, and 48 hours after operation from each groups. The extracted lipids were structurally identified based on collision-induced dissociation (CID) experiments and individual serums were quantitatively analyzed through selective reaction monitoring (SRM) mode. The identified lipids were 142 phospholipids, 23 sphingolipids, and 240 glycerolipids.
E-mail joyce103@yonsei.ac.kr