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|
| Type |
Oral Presentation |
| Area |
Oral Presentation of Young Analytical Chemists II |
| Room No. |
Room 321 |
| Time |
FRI 10:05-: |
| Code |
ANAL2.O-10 |
| Subject |
Multi-channel Microchip Electrophoresis for Rapid Screening of Glutathione S-transferase Polymorphism Genotyping |
| Authors |
Yucheng Sun, Seong Ho Kang1,*
Department of Chemistry, Kyung Hee University, Korea
1Department of Applied Chemistry, Kyung Hee University, Korea
|
| Abstract |
A voltage programming (VP)-based microchip electrophoresis (ME) method was developed to detect specific sizes of DNA fragments. The optimum condition was deliberated on a single-channel microchip and was then applied to a multi-channel microchip for simultaneous high-throughput detection. Glutathione S-transferase (GST) polymorphism genes (M1/M1 = 215 bp/215 bp, M1/T1 = 215 bp/480 bp, and T1/T1 = 480 bp/480 bp) extracted from human blood were amplified by polymerase chain reaction and were introduced into the multi-channel microchip. Target DNA molecules amplified by only 10 PCR cycles could be detected by the VP-based multi-channel ME, but not by slab gel electrophoresis. Besides, the migration time for ME was less than 15 s which was 700 times faster than SGE. Furthermore, internal control (312 bp) was added for further identification of target DNA. The presented modality was demonstrated to be effective and rapid for highly sensitive and high-throughput screening of GST genes. |
| E-mail |
sun.yc900911@gmail.com |
|
122nd General Meeting of the KCS