122nd General Meeting of the KCS

Type Oral Presentation
Area Oral Presentation of Young Analytical Chemists II
Room No. Room 321
Time FRI 09:30-:
Code ANAL2.O-3
Subject Dispersive Liquid-Liquid Microextraction based on Deep Eutectic Solvent for the HPLC-UV Determination of Resveratrol, Oxyresveratrol and Piceatannol from Wine
Authors Jongsung Noh, Seung-Ho Lee, Hyun-Woo Cho1, Seung Woon Myung*
Department of Chemistry, Kyonggi University, Korea
1Department of Natural Science Chemistry, Kyonggi University, Korea
Abstract Polyphenols are phytochemicals that exist in various plants and are beneficial to human health. These ingredients prevent aging, protect the skin from ultraviolet rays, improve brain health and prevent dementia. Resveratrol, oxyresveratrol and piceatannol in grapes are typical polyphenols, and grape wine also contains these ingredients. In this study, resveratrol, oxyresveratrol and piceatannol in wine were extracted by deep eutectic solvent based dispersive liquid-liquid microextraction (DLLME) and a method for quantification these polyphenols using high performance liquid chromatography - UV/vis spectroscopy was established. Parameters for sample clean up and concentration were optimized and verified. Experiments were carried out to obtain optimal parameters such as the types and volumes of extraction solvent and dispersive solvent, molar ratio of hydrogen bonding donor and hydrogen bonding acceptor, ratio of deep eutectic solvent and water, the pH effect, and the effect of salt. Chromatographic separation with HPLC/UV-vis was performed using the eclipse XDB-C18 (2.1 mm id × 150 mm length, 4.6 μm particle size) column and gradient elution mode using water and acetonitrile. The established method would be applied to wines sold in the market and monitored.
E-mail qsto@kyonggi.ac.kr