We have demonstrated a novel, facile, economic, and green synthetic protocol to produce gold nanoparticles (Au NPs) by using Schisandra chenesis fruit extract. The synthesized Au NPs were characterized using UV–VIS spectroscopy, transmission electron microscopy, scanning electron microscopy, X-ray diffraction (XRD), FTIR, and energy dispersive X-ray analysis (EDXA). The catalytic efficiency of synthesized Au NPs were studied for one-pot construction of chromeno[2,3-d]pyrimidin-2-yl)phenol derivatives via one-pot three component reaction.