123rd General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Exhibition Hall 2
Time 4월 19일 (금요일) 11:00~12:30
Code ANAL.P-239
Subject Development and validation of a sensitive Gas Chromatography-Combustion/isotope Ratio Mass Spectrometric method for anti-doping purposes
Authors Kang Mi Lee
Doping Control Center, Korea Institute of Science and Technology, Korea
Abstract The World Anti-Doping Agency (WADA) bans the use of anabolic steroids by athletes for sports performance enhancement. Synthetic copies of anabolic steroids are chemically identical to endogenous steroids, but there are small differences in the 13C to 12C ratios of the synthetic analogues. These differences can be measured using a gas chromatography-combustion isotope ratio mass spectrometry (GC-C/IRMS). However GC-C/IRMS analysis requires an extensive clean-up procedures and a large volume of sample because it can only determine the 13C/ 12C ratios of a pure compound. In consideration of limited amount of urine for anti-doping analysis, using large volume of urine can be an issue to meet the requirement sensitivity of IRMS. Therefore, acetylation derivatization is commonly recommended in GC-C/IRMS analysis because acetylated compounds show superior gas chromatographic behavior even acetylation requires much cautions on sample preparation. The aim of this study is to develop and validate a sensitive GC-C-IRMS analysis method using acetylation for anabolic steroids in human urine. The new method was combined with HPLC cleanup and allowed accurate and reproducible results enough to be successfully applied to urine samples that contain low concentration of steroids. Moreover the developed method was fully satisfied with WADA criteria.
E-mail rkdal-lee@kist.re.kr