|
Type |
Poster Presentation |
Area |
Analytical Chemistry |
Room No. |
Exhibition Hall 2 |
Time |
4월 19일 (금요일) 11:00~12:30 |
Code |
ANAL.P-239 |
Subject |
Development and validation of a sensitive Gas Chromatography-Combustion/isotope Ratio Mass Spectrometric method for anti-doping purposes |
Authors |
Kang Mi Lee Doping Control Center, Korea Institute of Science and Technology, Korea |
Abstract |
The World Anti-Doping Agency (WADA) bans the use of anabolic steroids by athletes for sports performance enhancement. Synthetic copies of anabolic steroids are chemically identical to endogenous steroids, but there are small differences in the 13C to 12C ratios of the synthetic analogues. These differences can be measured using a gas chromatography-combustion isotope ratio mass spectrometry (GC-C/IRMS). However GC-C/IRMS analysis requires an extensive clean-up procedures and a large volume of sample because it can only determine the 13C/ 12C ratios of a pure compound. In consideration of limited amount of urine for anti-doping analysis, using large volume of urine can be an issue to meet the requirement sensitivity of IRMS. Therefore, acetylation derivatization is commonly recommended in GC-C/IRMS analysis because acetylated compounds show superior gas chromatographic behavior even acetylation requires much cautions on sample preparation. The aim of this study is to develop and validate a sensitive GC-C-IRMS analysis method using acetylation for anabolic steroids in human urine. The new method was combined with HPLC cleanup and allowed accurate and reproducible results enough to be successfully applied to urine samples that contain low concentration of steroids. Moreover the developed method was fully satisfied with WADA criteria. |
E-mail |
rkdal-lee@kist.re.kr |
|