|
|
| Type |
Poster Presentation |
| Area |
Organic Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 18일 (목요일) 11:00~12:30 |
| Code |
ORGN.P-161 |
| Subject |
Melanosome targetable tyrosinase-selective ratiometric probe |
| Authors |
Sun Young Park, Min Hee Lee*
Department of Chemistry, Sookmyung Women's University, Korea
|
| Abstract |
Tyrosinase is an important biomarker of melanoma cancer because this enzyme overexpresses in melanoma cancer cells and it is considered as a promising target for cancer diagnosis and treatment. Tyrosinase is localized to the melanosomes in melanocytes. Here, we developed a morpholine-naphthalimide hybrid (1) as a ratiometric fluorescent probe that allows for a precise and quantitative analysis of tyrosinase activity. Probe 1 showed fluorescence changes at 460 and 560 nm for tyrosinase activity over other biologically relevant species, including metals, anions, and redox species. The kinetic parameters for the enzymatic reaction of probe 1 towards tyrosinase were determined by using Michaelis-Menten analysis. Also, inhibitory assay of tyrosinase activity was performed using 1 in the presence of kojic acid, a well-known tyrosinase inhibitor. As the concentration of kojic acid increases, the fluorescence ratio (I560 nm/I460 nm) was decreased. Now, confocal microscopy is in progress. We believe that probe 1 can provide a tyrosinase-dependent ratio fluorescent image and it is expected to be used for a real-time monitoring of tyrosinase activity in live melanoma cells. |
| E-mail |
1411437@sookmyung.ac.kr |
|
123rd General Meeting of the KCS