123rd General Meeting of the KCS

Type Poster Presentation
Area Organic Chemistry
Room No. Exhibition Hall 2
Time 4월 18일 (목요일) 11:00~12:30
Code ORGN.P-162
Subject Synthesis of coumarin-appended naphthalimide fluorescence probe for sensing and distinguishing hydrogen sulfide from other biothiols
Authors Shin A Yoon, Min Hee Lee*
Department of Chemistry, Sookmyung Women's University, Korea
Abstract Bitothiols, such as hydrogen sulfide (H2S), cysteine, homocysteine, and glutathione, play important role in biological systems. The abnormal levels are directly associated with various human diseases. In this work, coumarin-appended naphthalimide 1 was developed as a multi-sensing fluorescence probe that can discriminate H2S from other biothiols. Initially, probe 1 displayed a coumarin emission at 465 nm. However, in the presence of H2S, the 1 exhibited a new emission at 550 nm was emerged, while the initial emission at 465 nm was constant. By measuring the fluorescence intensities at 465 and 550 nm we could obtain a ratiometric fluorescence signal change towards H2S concentration. On the other hands, in the case of cysteine, homocysteine, and glutathione, probe 1 showed fluorescence enhancements at 500 nm. Therefore, we could distinguish the H2S from other biothiols by monitoring the fluorescence intensity (FI) at 465 nm and FI ratio (I630 nm/I465 nm). Moreover, the limits of detection for GSH and H2S were calculated to be 6.6 μM and 1.2 μM, respectively. Furthermore, the sensing mechanisms of probe 1 for H2S and other thiols were thoroughly investigated by using HPLC and ESI-Mass analyses.
E-mail dbstlsdk23@sookmyung.ac.kr