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Type |
Poster Presentation |
Area |
Organic Chemistry |
Room No. |
Exhibition Hall 2 |
Time |
4월 18일 (목요일) 11:00~12:30 |
Code |
ORGN.P-162 |
Subject |
Synthesis of coumarin-appended naphthalimide fluorescence probe for sensing and distinguishing hydrogen sulfide from other biothiols
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Authors |
Shin A Yoon, Min Hee Lee* Department of Chemistry, Sookmyung Women's University, Korea |
Abstract |
Bitothiols, such as hydrogen sulfide (H2S), cysteine, homocysteine, and glutathione, play important role in biological systems. The abnormal levels are directly associated with various human diseases. In this work, coumarin-appended naphthalimide 1 was developed as a multi-sensing fluorescence probe that can discriminate H2S from other biothiols. Initially, probe 1 displayed a coumarin emission at 465 nm. However, in the presence of H2S, the 1 exhibited a new emission at 550 nm was emerged, while the initial emission at 465 nm was constant. By measuring the fluorescence intensities at 465 and 550 nm we could obtain a ratiometric fluorescence signal change towards H2S concentration. On the other hands, in the case of cysteine, homocysteine, and glutathione, probe 1 showed fluorescence enhancements at 500 nm. Therefore, we could distinguish the H2S from other biothiols by monitoring the fluorescence intensity (FI) at 465 nm and FI ratio (I630 nm/I465 nm). Moreover, the limits of detection for GSH and H2S were calculated to be 6.6 μM and 1.2 μM, respectively. Furthermore, the sensing mechanisms of probe 1 for H2S and other thiols were thoroughly investigated by using HPLC and ESI-Mass analyses.
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E-mail |
dbstlsdk23@sookmyung.ac.kr |
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