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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-341 |
| Subject |
Quantification of Tumor-associated Circulating Free DNA with Atomic Force Microscopy
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| Authors |
Woo Cheol Shim, Joon Won Park*
Department of Chemistry, Pohang University of Science and Technology, Korea
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| Abstract |
Unlike conventional invasive biopsy method, non-invasive liquid biopsy has come into the spotlight to obtain information about cancer from present biomarkers such as circulating free DNA (cfDNA), exosomes, circulating tumor cells (CTCs) in patient`s blood. Especially various methods that detect genetic alterations of circulating free DNA (cfDNA) can be used for diagnosis and prognosis of cancer
There have been many endeavors to reveal correlation between cfDNA and cancer in the past few years. Here we propose a new methodology using atomic force microscopy (AFM) to quantify tumor-specific genetic aberration involved in methylation change of cfDNA with very low abundance.
Quantification of methylated cfDNA is under way by using MBD2 (methyl-CpG-binding domain protein2) having the ability to recognize symmetrically methylated single CpG and UHRF1 that recognizes hemimethylated CpG sites. This unprecedented liquid biopsy followed by AFM will be a great help for conquering cancer.
Reference
1. Crowley, E.; Di Nicolantonio, F.; Loupakis, F.; Bardelli, A., Liquid biopsy: monitoring cancer-genetics in the blood. Nat. Rev. Clin. Oncol. 2013, 10 (8), 472.
2. Lee, Y.; Kim, Y.; Lee, D.; Roy, D.; Park, J. W., Quantification of Fewer than Ten Copies of a DNA Biomarker without Amplification or Labeling. J. Am. Chem. Soc. 2016, 138 (22), 7075-7081.
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| E-mail |
Shimwc@postech.ac.kr |
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123rd General Meeting of the KCS