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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-342 |
| Subject |
Quantitative Analysis of LIMK1 in a Single Cell with Atomic Force Microscopy
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| Authors |
Ji-seon Lim, Joon Won Park*
Department of Chemistry, Pohang University of Science and Technology, Korea
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| Abstract |
Proteins are essential parts of organisms and participate in virtually every process within cells, performing a variety of functions. LIMK1 is a multifunctional protein and is involved in regulation of cell motility, cell cycle, cytokinesis and cellular morphology. LIMK1 also regulates neurite growth, synaptic stability, growth cone motility, axon formation through modulation of Golgi dynamics and neuronal differentiation. This protein stimulates axon growth and may play a role in brain development. In particular, LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome, which is a unique neurodevelopmental disorder characterized by severe defects in visuospatial cognition and long-term memory. Accordingly, quantitative analysis of LIMK1 and their distribution in a single cell is important to study the biological role of LIMK1 in neurons.
Conventional methods for protein analysis including western blotting, ELISA, and immunofluorescence have some problems that they have low detection limit and use fluorescent molecules. Therefore, atomic force microscopy (AFM) is a good candidate to overcome the hurdles. Using the mapping capability of AFM, it is possible to obtain a map the distribution of a specific protein in a naonometric resolution without modification or amplification. In this way, LIMK1 can be analyzed quantitatively in a single cell and their distribution can be mapped at high lateral resolution. |
| E-mail |
jslim@postech.ac.kr |
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123rd General Meeting of the KCS