We demonstrated the polarization- and wavelength-dependent defocused scattering properties of singe gold nanostars (AuNSs) with multiple long branches on their surfaces at the localized surface plasmon resonance (LSPR) wavelengths. As a function of the rotational angle of a polarizer, the defocused DF scattering intensities of single AuNS were periodically changed without changing in the doughnut-shaped scattering patterns at two LSPR excitation wavelengths. Furthermore, the characteristic doughnut-shaped defocused scattering pattern enabled to resolve the spatial field distributions of single dipoles on the same AuNS surface at two LSPR wavelengths. Finally, we could track real-time rotational dynamics of a AuNS rotating on a glass slide under defocused microscopy. Therefore, we provided a deeper understanding of the defocused scattering properties of single AuNSs with multiple long branches randomly extended on the surface.