123rd General Meeting of the KCS

Type Poster Presentation
Area Life Chemistry
Room No. Exhibition Hall 2
Time 4월 19일 (금요일) 11:00~12:30
Code LIFE.P-344
Subject Construction of Auto-Inducible Promoters for Recombinant Protein Production
Authors Hakbeom Kim, Young Kee Chae1,*
biophysical chemistry, Sejong University, Korea
1Department of Chemistry, Sejong University, Korea
Abstract The production of recombinant proteins in E. coli involves such factors as host strains, expression vectors, culture media, and induction methods. The typical approach to producing heterologous proteins consists of the followings: (1) insertion of the target gene into a suitable vector, (2) transformation of a strain specialized for protein production with the resulting plasmid DNA, (3) growth of the host in a suitable medium and induction of the protein production at a right moment, and (4) further growth to get the maximum yield. We constructed expression plasmids with auto-inducible promoters working in a host that is not specialized for protein production, and in a TB medium which does not contain any secret ingredient. This plasmid also contains the color indicator which turns red when protein production is successful. We hope that these plasmids will help academic and industrial researchers save their time and effort in a routine protein production procedure. This work has been supported by Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1D1A1A02017545).
E-mail ykchae@sejong.ac.kr