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| Type |
Oral Presentation |
| Area |
Oral Presentations for Young Scholars in Organic Division |
| Room No. |
Room 301+302 |
| Time |
THU 10:00-10:15 |
| Code |
ORGN.O-5 |
| Subject |
A New Approach to Polarity Probe for Quantitative Intracellular Imaging |
| Authors |
Sang Jun Park, Hwan Myung Kim*
Department of Energy Systems Research, Ajou University, Korea
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| Abstract |
Cell homeostasis is maintained by various factors such as viscosity, polarity, temperature and pH which controls the transport and diffusion of substances inside and outside the cell, and interactions between substances. Among them, intracellular polarity is an important parameter that promotes various cellular processes such as differentiation, local membrane growth, activation of the immune response, directional cell migration, and vector transport of molecules across the cell layer. The pathological activity of the cell changes the cell polarity, especially abnormal polarity is directly related to cancer. Thus, detecting of the cell polarity is essential for monitoring the status of each cell. The polarity of a typical solution can be detected with a commercial polarity meter. However, the only way to detect the intracellular polarity is detection using fluorescent probes. Most reported polarity probes have a turn-on system when they are protonated at intramolecular carbonyl groups in polar environment. Another system is ICT-based probes that is changed of the fluorescence wavelength due to polarity change. These probes are located in each organelle of the cells, showing the polarity of the microenvironment. We developed a ratiometric polarity probe with an intramolecular cyclization-opening system, depending on the polarity, unlike conventional methods. This probe is located in various intracellular regions as well as specific organelles in the cell and shows the distribution of the polarity of various regions within the cell. |
| E-mail |
goxgo81@ajou.ac.kr |
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123rd General Meeting of the KCS