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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-345 |
| Subject |
Mimicry of the Cytoskeleton; Actin-microtubule co-formation in a Giant Unilamellar Vesicle |
| Authors |
Sungwoo Jeong, ChangHo Kim1, Huong Thanh Nguyen2, Agustina Setiawati1, Kwanwoo Shin*
Department of Chemistry, Sogang University, Korea
1Sogang University, Korea
2Chemistry, Sogang University, Korea
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| Abstract |
Microtubule and actin cytoskeletons are physically contacted in a cell and dynamically coordinated to play vital roles in many cell functions, from migration, growth, and division. These structural dynamics of cytoskeletal proteins are of interest, the physical roles of cross-linking proteins between two filaments have been identified. Yet most studies were performed in a highly controlled interface or a bulk. Recently, we developed to simulate a cytoskeleton formation through ATP-dependent actin polymerization in a giant unilamellar vesicle. Optical stimulation initiated ATP synthesis and induced ATP-dependent actin polymerization, leading to the growth of three-dimensional highly curled actin filament network. In this study, we further added the ingredients for microtubule formation into the actin polymerizable GUV system and initiated the filament formation of those two cytoskeleton proteins simultaneously, We will discuss how these highly curled actin filaments in the single vesicle affect the structural environment in the presence of highly straight microtubule filaments. We will discuss how these two filaments are interacting in a highly confined, cell-like space where they are mutually restricted in further research. |
| E-mail |
jluke714@sogang.ac.kr |
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123rd General Meeting of the KCS