123rd General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Exhibition Hall 2
Time 4월 19일 (금요일) 11:00~12:30
Code ANAL.P-243
Subject Sensitive and Selective Detection of Symmetric Dimethylarginine by Competitive Enzyme-Linked Immunosorbent Assay
Authors Jimin Oh, Insook Rhee *
Department of Chemistry, Seoul Women's University, Korea
Abstract Sensitive immunoassay has the unique properties related to complex form of target and binder. It can be fitted to these requirements with simplicity, specificity and sensitivity to accomplish selective assay of target detection. A competitive enzyme-linked immunosorbent assay (ELISA) for detection of symmetric dimethylarginine (SDMA) in serum was developed using the SDMA-HRP conjugate, as a tracer, synthesized via activating the aldehyde groups of horseradish peroxidase (HRP). A various initial molar ratio of HRP:SDMA (1:20, 1:50 and 1:100) was applied. And then, we evaluate the sensitivity and selectivity of this assay. Firstly, to assess the sensitivity, we chose 1:100 ratio conjugate and observed the best sensitivity with the LOD: 1.15×10-10 M. Secondly, to appraise the selectivity, we used various cross-reactants having similar molecular structure to SDMA such as asymmetric dimethylarginine (ADMA), methyl arginine and L-arginine. This new method shows high selectivity for SDMA and none of the cross-reactants show the cross-reactivity. SDMA, the structural isomer of ADMA, is a methylated derivative of the amino acid L-Arginine and is strictly eliminated by renal extraction. In the past, creatinine was the most common bio-marker of renal function, especially glomerular filtration rate (GFR). However, SDMA has been shown more sensitive than creatinine because of the imperviousness to the physical factors and the earlier increasing with chronic kidney disease (CKD). Thus, we expect that this developed method can be a novel approaches for the determination of SDMA and early diagnosis of CKD.
E-mail 95127jimin@naver.com