123rd General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Exhibition Hall 2
Time 4월 19일 (금요일) 11:00~12:30
Code ANAL.P-246
Subject Comprehensive analysis of polyglycerophospholipids by isotope-labeled methylation method
Authors JongCheol Lee, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Abstract Polyglycerophospholipids (PGPLs) refer to phospholipids (PLs) having two or three glycerol moieties and are generally synthesized from phosphatidylglycerol (PG) as a precursor. PG, bis(monoacylglycero)phosphate (BMP), hemi BDP, and bis(diacylglycero)phosphate (BDP) with two glycerols, and dilysocardiolipin (DLCL), monolysocardiolipin (MLCL), and cardiolipin (CL) with three glycerols belong to PGPLs. Since these lipids are closely related to each other and regarded as biomarkers with high selectivity in various diseases rather than other PLs, simultaneous analysis of PGPLs is required to understand their pathological roles. In this study, methylation of PGPL was introduced to increase the improvement of separation and MS detection of PGPLs and their regiosisomers, and then the isotope labeled methylation (ILM) method was applied for the relative quantitations of PGPLs using nUHPLC-ESI-MS/MS. The efficiency of methylation for PGPLs was higher than > 97% for all of species and ILM method enhanced the S/N ratio of most PGPLs including newly identified species (regioisomers of hemi BDP, DLCL, and MLCL), which were not detected before methylation. The molecular structures of regioisomers can be distinguished by their retention time differences and characteristic fragment ion spectra. When SH-SY5Y cells with and without the drug treatements to induce Parkinson’s disease model were analyzed by ILM method, a total of 230 PGPLs including 16 hemi BDP, 71 MLCL and 121 CL species were identified, which is the record breaking number of analyzed PGPLs so far. Furthermore, seven CL species having arachidonic or docosahexanoic fatty acid chains were found to be up-regulated upon drugs treatment, which is an indicative of CL remodeling process in Parkinson’s disease model.
E-mail jchul@yonsei.ac.kr