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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
ANAL.P-247 |
| Subject |
Separation of plasma exosomes and lipoproteins using frit-inlet asymmetrical flow field-flow fractionation with multi-angle light scattering |
| Authors |
Young Beom Kim, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
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| Abstract |
Recently, exosomes are of a great interests due to the possible roles in developing biomarkers for disease diagnosis and prognosis. Exosomes are extracellular vesicles (30 – 150 nm) that are found in blood, urine, and biological fluids, and play important roles in cell signaling. Lipoproteins in blood which carry lipids throughout the body are classified as high-density lipoprotein (HDL, 5 – 15 nm), low-density lipoprotein (LDL, 18 – 28 nm), and very low density lipoproteins (VLDL, 30 – 80 nm). However, it is challenging to isolate exosomes from blood sample due to the presence of lipoproteins. . Field-flow fractionation (FFF) is an analytical technique that separate particles from nano to micron size in an open channel space without packing materials, which is advantageous for separating biomolecules. This study attempts to separate exosomes and lipoproteins in blood plasma samples by frit-inlet asymmetrical flow field-flow fractionation (FI-AF4) using field programming with a UV detection (UV) and multi-angle light scattering (MALS) detections. Exosomes and lipoproteins that were fractionated by FI-AF4 were identified by Western blot and transmission electron microscope (TEM). |
| E-mail |
bum5203@yonsei.ac.kr |
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123rd General Meeting of the KCS