123rd General Meeting of the KCS

Type Poster Presentation
Area Polymer Chemistry
Room No. Exhibition Hall 2
Time 4월 19일 (금요일) 11:00~12:30
Code POLY.P-14
Subject Difference between anisotropic and isotropic particles in flow cytometry
Authors Hoyeon Lee, Daewon Sohn1,*
Graduate student in chemistry, Hanyang University, Korea
1Department of Chemistry, Hanyang University, Korea
Abstract As a matter of shapes, most of bacteria have the rod-like shape, whereas red blood cells (RBCs) have the disk-like structure. This research was focused on increasing sensitivity for bacteria exclusively. We could discover the shape difference of bacteria and RBCs by using light scattering technique in flow cytometry. Two different silica particles were used representing bacteria and RBCs. Dependence on the shape of particles for signal intensity has been investigated. Isotropic sphere silica particles representing human red blood cells with three different sizes and anisotropic rod-like silica particles representing bacteria were synthesized. These materials were analyzed with a dynamic light scattering (DLS) and scanning electron microscope (SEM). Samples containing sphere or rod-like particles themselves or their mixtures were measured by using a flow cytometer for the height data of forward scatter (FSC-H), which means intensity of signals. Although radius of gyration of rod-like particles is bigger than sphere particles, it has weaker intensity. The anisotropy of rod-like particles makes this kind of difference. Since sphere particles are isotropic, these particles have the round-shaped scattering pattern. And this isotropic property can keep most scattered signals within the detecting area which can collect signals in practice. In contrast, anisotropic particles form two symmetric lobes when scattering. And the edge of this lobe escape detecting area. In addition, flow cytometry experiments with different ratios of these two particles were also carried out for sensitivity of detection on anisotropic rod-like silica particles. rod-like particles are observed even when it was only about a tenth of round-shaped particles.
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