|
Type |
Oral Presentation |
Area |
Oral Presentation of Young Life Chemist |
Room No. |
Room 303 |
Time |
THU 10:45-11:00 |
Code |
LIFE.O-8 |
Subject |
Seeing DNA Sequences: Fluorescent Polypyrrole for A/T Sequence Visualization on Single DNA Molecules |
Authors |
Seonghyun Lee, Kyubong Jo* Department of Chemistry, Sogang University, Korea |
Abstract |
Fluorophore-linked, sequence-specific DNA binding reagents can visualize sequence information on a large DNA molecule. In this talk, we synthesized newly designed TAMRA-linked polypyrrole to visualize adenine and thymine base pairs. A fluorescent image of the stained DNA molecule generates an intensity profile based on A/T frequency, revealing a characteristic sequence composition pattern. Computer-aided comparison of this intensity pattern with the genome sequence allowed us to determine the DNA sequence on a visualized DNA molecule from possible intensity profile pattern candidates for a given genome. Moreover, TAMRA-polypyrrole offers robust advantages for single DNA molecule detection: no fluorophore-mediated photocleavage and no structural deformation, since it exhibits a sequence-specific pattern alone without the use of intercalating dyes such as YOYO-1. Accordingly, we were able to identify genomic DNA fragments from E. coli cells by aligning them to the genomic A/T frequency map based on TAMRA-polypyrrole-generated intensity profiles. |
E-mail |
shlee@sogang.ac.kr |
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