123rd General Meeting of the KCS

Type Poster Presentation
Area Material Chemistry
Room No. Exhibition Hall 2
Time 4월 19일 (금요일) 11:00~12:30
Code MAT.P-435
Subject Biomimetic Signal Transduction Cascade using Pro-Nanozyme for Enzyme Activity Assay
Authors Minsoo Kim, Dongkap Kim1, Jaegeun Noh*, Youngdo Jeong1,*
Department of Chemistry, Hanyang University, Korea
1Biomedical Research Institute, Korea Institute of Science and Technology, Korea
Abstract In the intracellular signaling system, initial stimulus amplifies huge signals through the chemical cascade reactions called signaling transduction pathway, activating related pro-enzymes. However, mimicking the signal transduction cascades for biosensor systems is challenging due to the complex design of the artificial pro-enzyme that can be activated by specific stimuli. Herein, we present the sequential signal transduction for enzyme activity assay using the amphiphilic nanozyme and the biphasic reaction system. In the water layer, when one of the nanozymes that loaded hydrophobic catalysts recognizes the specific signal, it delivers the tens of the catalysts to the oil layer including pro-fluorophore molecules, transducing the stimuli to the catalysts. The delivered catalysts convert the pro-fluorophore to the fluorophore, amplifying the catalytic signal to the optical signal. The potential of this assay mimicking intracellular signal transduction cascade was demonstrated through the monitoring of activity of Caspase 3, establishing the artificial signal transduction platform for both diagnosis and drug screening.
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