Recently, the control of supramolecular polymerization process has attracted attention to many researchers. We designed a series of bisporphyrin derivatives (DPP, TPP, mTPP), composed of two zinc porphyrin units bridged by amide groups and diacetylene moiety with long alkyl side chains. All three bisporphyrins were self-assembled in methylcyclohexane (MCH) at low temperature (273 K) through hydrogen bonding and π-π stacking. Unlike DPP and mTPP, TPP show distinct different aggregation mode upon preparation method; UV/Vis absorption spectra show that J-aggregates and H-aggregates were formed from fast and slow cooling of hot MCH solution, respectively. UV/Vis absorption spectral changes of TPP J-aggregate clearly exhibited the transformation from J-aggregate to H-aggregates. The transformation process of J-aggregates was visualized through atomic force microscopy (AFM) and these AFM images show helical assembled nanofibers of TPP. Through chiral-auxiliary approaches to this transformation process, we can control the helicity of aggregated nanohelices.