123rd General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Exhibition Hall 2
Time 4월 19일 (금요일) 11:00~12:30
Code ANAL.P-259
Subject HPLC-UV Determiation of Resveratrol, Oxyresveratrol and Piceatannol from Wine
Authors Jongsung Noh, JinSol Lee, Won Hoe Koo, Hyun-Woo Cho1, Seung Woon Myung*
Department of Chemistry, Kyonggi University, Korea
1Department of Natural Science Chemistry, Kyonggi University, Korea
Abstract Polyphenols are phytochemicals that exist in various plants and are beneficial to human health. These ingredients prevent aging, protect the skin from ultraviolet rays, improve brain health and prevent dementia. Resveratrol, oxyresveratrol and piceatannol in grapes are typical polyphenols, and grape wine also contains these ingredients. In this study, resveratrol, oxyresveratrol and piceatannol in wine were extracted by deep eutectic solvent based dispersive liquid-liquid microextraction (DES-DLLME) and a method for quantification these polyphenols using high performance liquid chromatography - UV/vis spectroscopy was established. Parameters for sample clean up and concentration were optimized and verified. Experiments were carried out to obtain optimal parameters such as the types and volumes of extraction solvent and dispersive solvent, volume of acetic, anhydride, time of derivatization, type of basic solution for derivatization, and the effect of salt. Chromatographic separation with HPLC/UV-vis was performed using the Innoval ODS-2 (4.6 mm id × 150 mm length, 5 μm particle size) column and gradient elution mode using water and methanol.
E-mail qsto@kyonggi.ac.kr