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|
| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
ANAL.P-261 |
| Subject |
Development and validation of anabolic steroids screening method for anti-doping with gas chromatography/tandem mass spectrometry |
| Authors |
Yinglan Xu, Kang Mi Lee, Jaeick Lee, Junghyun Son, Oh-Seung Kwon*
Doping Control Center, Korea Institute of Science and Technology, Korea
|
| Abstract |
Anabolic androgenic steroids (AAS) are the major class of doping agents and their consumption produces adverse effects involving several organs and system diseases. The World Anti‐Doping Agency (WADA) encourages drug testing laboratories to develop anabolic steroids screening methods which can determine a large number of compounds at very low detection levels in urine. In this study, new screening method for the urinary detection of anabolic steroids has been developed and validated using gas chromatography/tandem mass spectrometry (GC/MS/MS). The method takes advantage of enhancing selectivity, sensitivity and efficiency compare to using gas chromatography mass spectrometry (GC/MS). More than 100 substances including endogenous and exogenous steroids could finally be monitored in 16 min with low concentrations in accordance with the minimum required performance levels of the World Anti-Doping Agency. The selectivity of the method showed that no interfering peaks were observed at the retention time of the analytes. Moreover, most of the compounds showed highly satisfactory intermediate precision (< 25%). The optimized method was successfully applied to doping analysis of PyeongChang winter olympic games. |
| E-mail |
xyl@kist.re.kr |
|
123rd General Meeting of the KCS