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| Type |
Poster Presentation |
| Area |
Life Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
LIFE.P-360 |
| Subject |
Multi-component Extracellular Matrix (ECM) Formation for 3D Cell Culture |
| Authors |
Agustina Setiawati, Huong Thanh Nguyen1, Sungwoo Jeong2, ChangHo Kim3, Kwanwoo Shin2,*
Chemistry, Sogang University, Sogang University, Korea
1Chemistry, Sogang University, Korea
2Department of Chemistry, Sogang University, Korea
3Institute of Biological Interfaces, Sogang University, Korea
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| Abstract |
Extracellular matrix (ECM) protein networks provide microenvironment which determine cell differentiation, proliferation, signalling and molecular pathway. By using ECM network, 3D cell culture is feasible to be generated to mimic in vivo cellular behaviour. However, the architecture and chemical components are greatly variable and tissue specific. In this study, we applied microcontact printing of fibronectin as a template of controllable cellular fibronectin network formation for 3D cell culture application.
We fabricated micro contact printing of fibronectin and cultured fibroblast on it. The FN patterned network then decellularized by freeze thawing method. Finally, the cells was reseeded on FN network and this step is repeated until several layers of cells grow on the FN-FN network specifically.
Our method succesfully generated controllable FN-FN network from cells. Patterned FN-FN network then reseeded by second layer of fibroblast and maintain its viability for more than two weeks. Therefore, this ECM network has high potency to be applied for in vivo mimicking cell culture.
Keywords: fibronectin, ECM, microcontact printing, 3D cell culture
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| E-mail |
nina.lifesci@gmail.com |
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123rd General Meeting of the KCS