123rd General Meeting of the KCS

Type Poster Presentation
Area Analytical Chemistry
Room No. Exhibition Hall 2
Time 4월 19일 (금요일) 11:00~12:30
Code ANAL.P-269
Subject Superlocalization of Single Tumor Necrosis Factor-α Molecules on Nano-Region Using 3D Enhanced Dark-Field Nanoscopy
Authors Seungah Lee, Dongho Jang, Seong Ho Kang*
Department of Applied Chemistry, Kyung Hee University, Korea
Abstract Superlocalization on nano-region by the least-cubic algorithm of biomarker was performed using three dimensional (3D) enhanced dark-field nanoscopy. The tumor necrosis factor-α (TNF-α) with nanotag (silver) as a biomarker was immunoreacted on 100 nm gold nanospot. The localization precision and sensitivity with a least-cubic algorithm provided accurate position information for the immunotargeted site on 100 nm gold spot. The accurate localization of the nanoparticle for detection (i.e., silver and gold) after reaction provided important information to reduce the error in data interpretation as well as the presence or absence of reaction. Especially, negligible signals were observed using interfering proteins without cross-reactivity, showing the high specificity of the system for single biomolecule detection.
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