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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
ANAL.P-276 |
| Subject |
Proteomics Approach for Plasma Exosomes in Lung Cancer Patients |
| Authors |
Jihoon Shin, Jinwook Lee, Min-gyu Youn, Miseon Jeong, Junghoon Kang, Wonryeon Cho1,*
Department of Chemistry, Wonkwang University, Korea
1Department of Bio-nanochemistry, Wonkwang University, Korea
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| Abstract |
Exosomes are cell-derived nanovesicles that are rich source of novel biomarkers in the diagnosis and prognosis of disease. Exosomes have highly-specialized functions and play a key role in processing intercellular signaling. They are present in many complex biological fluids such as plasma. This study intends to identify exosome biomarker from lung cancer plasma. Exosomes were individually extracted from 46 patients’ plasma of lung cancer through size-exclusion chromatography. Each exosome sample was pooled into three TNM groups. Each group of exosome samples from lung cancer patients was lysed with RIPA buffer. After Lysis, separated proteins from exosome were trypsin digested. Each group samples were identified nLC-MS/MS using Orbitrap Elite multiple times with various methods for exosomal protein identification from lung cancer plasma. |
| E-mail |
ioi09@wku.ac.kr |
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123rd General Meeting of the KCS