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| Type |
Poster Presentation |
| Area |
Analytical Chemistry |
| Room No. |
Exhibition Hall 2 |
| Time |
4월 19일 (금요일) 11:00~12:30 |
| Code |
ANAL.P-278 |
| Subject |
A resistance study of serum-starvated neuroblatoma cell at specific drug concentration |
| Authors |
Sooyeon Chae, Jong Yoon Han, Chae Eun Heo, Min Gyeongseo, MyungKook Son, Min Ji Kim, Hugh I. Kim, Chae Ri Park, Dongjoon Im, Chaehyeon Yoon, Hugh I. Kim*
Department of Chemistry, Korea University, Korea
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| Abstract |
Neuroblastoma is a solid tumor that arises from the developing sympathetic nervous system. As neuroblastoma belongs to heterogeneous disease, designing effective treatment for each patient is challenging. To examine individual variation in drug response, drug stability and efficiency tests are commonly performed in 2-dimensional (2D) cell or xenograft before clinical trial. However, additional condition to in vitro systems is crucial to closely mimic the complexity of in vivo system. Serum starvation is one of the most frequently used methods to imitate in vivo microenvironment. Thus, examining drug efficacy and resistance of serum-starved 2D cell has been suggested as a screening strategy for individual therapy. However, the differences between serum-starved and normal cells have not been fully understood.
In this study, we studied the drug resistance in cancer cell depending on serum starvation. Especially, Topotecan, one of the anticancer drugs which inhibit DNA synthesis, was used. We observed that neuroblastoma cells in serum starvation show the resistance when they are treated with 1 μM topotecan. Then, quantitation of drug uptake using reverse phase liquid chromatography-triple quadrupole mass spectrometry were performed. As a result of the quantitation of drug uptake, Serum starvation conditions showed relatively lower uptake at 48 hours when chemoresistance occurs. In correlation to distinct change in cellular uptake, we also performed the proteomic analysis to find differentiated protein group related to resistance in serum starvation. The fundamental understanding of anticancer drugs resistance on serum starvation can provide prediction of strategy for individual therapy.
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| E-mail |
dkfvk35@naver.com |
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123rd General Meeting of the KCS